19 Feb 2016 --- A new method developed in Finland efficiently separates proteins from agrobiomass by-products – specifically brewer's spent grain (BSG).
According to research by the Technical Research Center of Finland (VTT), extraction with deep eutectic solvents (DESs) offer an efficient, sustainable and easy method for dissolving proteins from agrobiomass by-products.
What are DESs?
DESs are mixtures of solids that form a liquid solution at low temperatures when mixed in suitable ratios. A DES incorporates two or more substances with high melting points into a mixture with a melting point substantially lower than any of the inpidual pure components. A known example of this is the mixture (molar ratio 1:2) of choline chloride (mp 302 °C) and urea (mp 133 °C), which has a melting point of 12 °C. DESs represent a new generation of organic solvents; research into their possible applications only began in recent years.
DESs are used in metal extraction and polishing, but today, they are also used in the separation of biomass fractions. Previously, there has been no research on how well they are suited to separating proteins from biomass.
DESs have nutritional value; they contain, for example, a precursor of vitamin B used in poultry feed. Therefore, the method may also be suitable for the production of a protein concentrate to be added to food, though this requires further study. According to VTT research scientists, animal feed is the primary application for protein enriched with DESs.
Easy and efficient dissolving process
The method used is simple: DES and solid biomass, such as BSG, are heated and mixed in the reactor for two hours. Finally, water is added into the reactor and the solids separated by filtering. DES molecules, proteins and water are separated through membrane filtering. The protein is then dried, and the end result is a high-quality protein concentrate that could be utilised in, for example, the feeding of pigs or poultry.
The method has been tested on separating protein from BSG, rapeseed press cake and wheat bran, all of which contain significant amounts of protein. These food industry by-products contain significant amounts of fiber, which decreases their suitability as feed for production animals that are not ruminants.
BSG responded best to protein separation with DES: almost 80 percent of the protein could be separated, whilst conventional extraction methods can achieve no more than 40 percent. The separation of other substances, such as carbohydrates, can be optimised through the choice of DES.
This new protein enrichment method can particularly benefit breweries and animal feed producers, but there are hopes that after further research, this method could also find applications in the food industry.
Protein in rapeseed press cake could also be separated to a high degree, but the difference with traditional extraction methods was not significant. Further research is required with regard to wheat bran. Separating protein from wheat bran is known to be difficult.
VTT has tested the method with a 60-litre pilot system. The method can be easily scaled up to an industrial scale. The chemicals used are cheap and suitable for use in foods, and many are FDA-approved.
The commercialisation of this patented invention is currently being examined. The research results will be published in a scientific journal in 2016.
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